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TBE or Tris/Borate/EDTA, is a buffer solution containing a mixture of Tris base, boric acid and EDTA. In molecular biology, TBE and TAE buffers are often used in procedures involving nucleic acids, the most common being electrophoresis. Tris-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water. EDTA is a chelator of divalent cations, particularly of magnesium (Mg2+). As these ions are necessary co-factors for many enzymes, including contaminant nucleases, the role of the EDTA is to protect the nucleic acids against enzymatic degradation. But since Mg2+ is also a co-factor for many useful DNA-modifying enzymes such as restriction enzymes and DNA polymerases, its concentration in TBE or TAE buffers is generally kept low (typically at around 1 mM). More recently discovered substitutes for TBE and TAE buffers for electrophoresis are available. ==Recipe (1 liter of 5X stock solution)== *54 g of Tris base (CAS# 77-86-1) *27.5 g of boric acid (CAS# 10043-35-3) *20 ml of 0.5 M EDTA (CAS# 60-00-4) (pH 8.0) Adjust pH to 8.3 by HCl.〔https://www.eeb.ucla.edu/Faculty/Barber/Web%20Protocols/Protocol7.pdf〕 TBE can be diluted to 1X prior to use in electrophoresis, 0.5x is acceptable as well. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「TBE buffer」の詳細全文を読む スポンサード リンク
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